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KMID : 0613820190290070779
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Journal of Life Science
2019 Volume.29 No. 7 p.779 ~ p.784
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Evaluation of Antioxidant Fractions and Hair Loss Prevention Effects of Platycodon grandiflorum
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Jung Min-Hwa
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Abstract
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Free radicals are known to inhibit hair vitality by damaging the cell membranes of the hair follicles. The purpose of this study was to determine the antioxidant activities and the capacity for hair loss prevention of extracts from Platycodon grandiflorum. We prepared butanol (BF) and water (WF) fractions from P. grandiflorum. DPPH and ABTS radical scavenging activities were measured to investigate the antioxidant activities of the fractions. Both fractions exhibited dose-dependent antioxidant activities for DPPH radical production, and BF and WF almost completely suppressed ABTS radical production when supplied at 10 and 100 mg/ml, respectively. We confirmed a skin regeneration effect by treating human HaCaT skin cells with a range of BF and WF concentrations for 24 and 48 hr. The extract treatments accelerated cell proliferation. We also assayed the capacity of BF and WF to suppress inflammation using RAW264.7 cells. BF dose-dependently suppressed nitrous oxide (NO) production. Treatment of human hair follicle dermal papilla cells (HFDPC) with BF and WF promoted cell proliferation after 24, 48, and 72 hr of treatment when supplied at 10, 50, 100, and 200 μg/ml. Taken together, these results confirm the possibility of using BF and WF extracts from P. grandiflorum in formulating hair loss prevention products.
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KEYWORD
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Antioxidant, DPPH, hair loss, HFDPC cell, Platycodon grandiflorum
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